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os.unlink(path)

#!/usr/bin/python3

import os, sys

# List directory
print ("Directory is: %s" % os.listdir(os.getcwd()))

os.unlink("aa.txt")

# Directory after deletion
print ("Directory after deletion: %s" % os.listdir(os.getcwd()))

Directory is:
['a1.txt', 'aa.txt', 'resume.doc']
Directory after deletion:
['a1.txt', 'resume.doc']