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fileObject.write( [ str ])

1:www.tutorialpro.org
2:www.tutorialpro.org
3:www.tutorialpro.org
4:www.tutorialpro.org
5:www.tutorialpro.org

#!/usr/bin/python3

# Open the file
fo = open("tutorialpro.txt", "r+")
print("File name: ", fo.name)

str = "6:www.tutorialpro.org"
# Write a line at the end of the file
fo.seek(0, 2)
line = fo.write(str)

# Read the entire file content
fo.seek(0,0)
for index in range(6):
    line = next(fo)
    print("File line number %d - %s" % (index, line))

# Close the file
fo.close()

File line number 0 - 1:www.tutorialpro.org
File line number 1 - 2:www.tutorialpro.org
File line number 2 - 3:www.tutorialpro.org
File line number 3 - 4:www.tutorialpro.org
File line number 4 - 5:www.tutorialpro.org
File line number 5 - 6:www.tutorialpro.org

$ cat tutorialpro.txt 
1:www.tutorialpro.org
2:www.tutorialpro.org
3:www.tutorialpro.org
4:www.tutorialpro.org
5:www.tutorialpro.org
6:www.tutorialpro.org