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fileObject.seek(offset[, whence])

>>> f = open('workfile', 'rb+')
>>> f.write(b'0123456789abcdef')
16
>>> f.seek(5)      # Move to the sixth byte of the file
5
>>> f.read(1)
b'5'
>>> f.seek(-3, 2)  # Move to the third-to-last byte of the file
13
>>> f.read(1)
b'd'

1:www.tutorialpro.org
2:www.tutorialpro.org
3:www.tutorialpro.org
4:www.tutorialpro.org
5:www.tutorialpro.org

#!/usr/bin/python3

# Open file
fo = open("tutorialpro.txt", "r+")
print ("File name: ", fo.name)

line = fo.readline()
print ("Data read: %s" % (line))

# Reset file read pointer to the beginning
fo.seek(0, 0)
line = fo.readline()
print ("Data read: %s" % (line))

# Close file
fo.close()

File name:  tutorialpro.txt
Data read: 1:www.tutorialpro.org

Data read: 1:www.tutorialpro.org