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fileObject.readline();

1:www.tutorialpro.org
2:www.tutorialpro.org
3:www.tutorialpro.org
4:www.tutorialpro.org
5:www.tutorialpro.org

#!/usr/bin/python3

# Open file
fo = open("tutorialpro.txt", "r+")
print("File name: ", fo.name)

line = fo.readline()
print("First line read: %s" % (line))

line = fo.readline(5)
print("String read: %s" % (line))

# Close file
fo.close()

File name:  tutorialpro.txt
First line read: 1:www.tutorialpro.org

String read: 2:www