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int imagecolorexactalpha ( resource $image , int $red , int $green , int $blue , int $alpha )

<?php

// Create an image
$im = imagecreatefrompng('tutorialpro-logo.png');

$colors   = Array();
$colors[] = imagecolorexactalpha($im, 255, 0, 0, 0);
$colors[] = imagecolorexactalpha($im, 0, 0, 0, 127);
$colors[] = imagecolorexactalpha($im, 255, 255, 255, 55);
$colors[] = imagecolorexactalpha($im, 100, 255, 52, 20);

print_r($colors);

// Free from memory
imagedestroy($im);
?>

Array
(
    [0] => 16711680
    [1] => 2130706432
    [2] => 939524095
    [3] => 342163252
)